Human Platelet Actin

Human platelet actin. Isolation and properties.

Mechanism of the interaction of human platelet profilin with actin

We have reexamined the interaction of purified platelet profilin with actin and present evidence that simple sequestration of actin monomers in a 1:1 complex with profilin cannot explain many of the effects of profilin on actin assembly. Three different methods to assess binding of profilin to actin show that the complex with platelet actin has a dissociation constant in the range of 1 to 5 mic...

Glycoprotein Ib- and actin-binding regions in human platelet actin-binding protein.

Introduction The cytoskeleton in human platelets plays an important role in the dynamic changes that occur during platelet adhesion, shape change and clot retraction. The actin filaments in the cytoskeleton are cross-linked into three-dimensional networks by actin-binding protein (ABP or filamin). The cytoskeleton is linked to the platelet plasma membrane by an association between ABP and a tra...

Human platelet antigen polymorphism in bronchial asthma

Background: Asthma is recognized as a common cause of disability, of great economic cost, and of preventable deaths. In this study we aimed to test our hypothesis to evaluate the relationship between the Human Platelet Antigen-1(HPA-1) polymorphism and bronchial asthma and its severity, which would suggest genetic variances that may be responsible for expression or activation of these receptors...

Phosphorylation of platelet actin-binding protein during platelet activation.

In this study we have followed the 32P-labeling of actin-binding protein as a function of platelet activation. Utilizing polyacrylamide-sodium dodecyl sulfate gel electrophoresis to resolve total platelet protein samples, we found 2--3-fold labeling increases in actin-binding protein 30--60 sec after thrombin stimulation. Somewhat larger increases were observed for 40,000 and 20,000 apparent mo...